Part:BBa_J100032:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J100032

Tube A: E. coli + plasmids containing proUP3 (experimental)
Tube B: E. coli + plasmids containing proUP3 + 20µL of 500mM NaCl (experimental #1 + inducer)
Tube C: E. coli + plasmids containing the placeholder J100028 (negative control)
Tube D: E. coli + plasmids containing pTet (positive control)
Tube E: E. coli + plasmids containing pLux + 20µL HSL-3OC6 (experimental #2 + inducer)
Tube F: E. coli + plasmids containing pLux (experimental #2)

The proUP3 promoter appeared to function, given the data in tube A above. However, contrary to expectations, it did not function in the presence of the osmotic inducer as shown in the data for tube B. This was likely due to the high concentration of NaCl supplied in addition to the LB, which already had a relatively high saline concentration. Further experimentation observing the promoter in the presence of varying concentrations of NaCl could be useful to understand more about this functioning promoter.

User Reviews

UNIQf1adf6c599404851-partinfo-00000000-QINU UNIQf1adf6c599404851-partinfo-00000001-QINU